Vibrio harveyi

prokaryotic facultative-anaerobic gram-negative halophilic

About

This bacteria is an advantageous good engineerable chassis because it can respond to several quorum sensing molecule.

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1578700/
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285556/

Growth Characteristics

As Vibrio harveyi is a bacteria found in sea water, it can not survive without salt in the medium. LB can be enough but Luria Marine is better.

Transformation Protocols

  1. Triparental conjugation: https://pub.uni-bielefeld.de/publication/1659456

Biobrick Parts

  1. LacI-mRFP: http://parts.igem.org/Part:BBa_J04450

Vectors

  1. pRK2073 helper plasmid
  2. pBBR1MCS5 - genta conjugative plasmid:

Acknowledgements

This entry was curated by iGEM 2017 team INSA-UPS France