Saccharomyces cerevisiae

eukaryotic facultative-anaerobic fungal yeast

Growth Characteristics

References:

  1. https://www.atcc.org/Products/All/204508.aspx#culturemethod

Culture Sources

  1. ATCC: https://www.atcc.org/Products/All/204508.aspx#culturemethod

Maintenance Protocols

  1. Growth and Maintnenance protocol: https://link.springer.com/protocol/10.1385%2F1-59259-210-4%3A009

Transformation Protocols

  1. High Efficiency Transformation: https://openwetware.org/wiki/High_Efficiency_Transformation

Other Protocols

  1. Cell Cycle Arrest: https://openwetware.org/wiki/Cell_Cycle_Arrest

  2. Assaying Mating: https://openwetware.org/wiki/Assaying_mating

  3. DNA prep: https://openwetware.org/wiki/Yeast_DNA_Prep

  4. Colony PCR: https://openwetware.org/wiki/Yeast_Colony_PCR

Biobrick Parts

  1. BBa_K401001: http://parts.igem.org/Part:BBa_K401001

  2. BBa_K801000: http://parts.igem.org/Part:BBa_K801000

  3. BBa_K2082208: http://parts.igem.org/Part:BBa_K2082208

  4. BBa_K2118002: http://parts.igem.org/Part:BBa_K2118002

Vectors

  1. pYAC4 (Burke87): https://openwetware.org/wiki/Yeast_artificial_chromosomes

  2. pJS97 / pJS98 plasmids (Shero91): https://openwetware.org/wiki/Yeast_artificial_chromosomes

  3. pCGS966 (Smith90, Smith92, Moir93) : https://openwetware.org/wiki/Yeast_artificial_chromosomes

  4. pRML1 / pRML2 (Spencer93) : https://openwetware.org/wiki/Yeast_artificial_chromosomes

Metabolic Models

  1. iMM904: http://bigg.ucsd.edu/models/iMM904

  2. iND750: http://bigg.ucsd.edu/models/iND750