Pichia pastoris

eukaryotic facultative-anaerobic fungal yeast

Culture Sources

  1. ATCC 28485:https://www.atcc.org/~/ps/28485.ashx

  2. NCYC 175: https://catalogue.ncyc.co.uk/komagataella-pastoris-175

  3. YPD: https://tools.thermofisher.com/content/sfs/manuals/easyselect_man.pdf

Maintenance Protocols

  1. Temperature: 24°C to 26°C Atmosphere: Typical aerobic growth media: YEPD, YM agar or broth, Emmons' modification of Sabouraud's agar: https://www.atcc.org/~/ps/28485.ashx

Transformation Protocols

  1. Pichia Yeast Transformation protocol - iGEM 2012: http://2012.igem.org/wiki/images/0/02/Pichia_Yeast_Transformation_protocol.pdf

  2. Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504082/

  3. High efficiency transformation by electroporation of Pichia pastoris: https://www.biotechniques.com/multimedia/archive/00003/BTN_A_05381BM04_O_3873a.pdf

Biobrick Parts

  1. pPICZa: http://parts.igem.org/Part:BBa_K805020

  2. Leucrocine I AMP + afactor: http://parts.igem.org/Part:BBa_K2278022

  3. cOT2 AMP + afactor: http://parts.igem.org/Part:BBa_K2278023

  4. D-NY15 AMP + afactor: http://parts.igem.org/Part:BBa_K2278021

  5. AOX1 promoter: http://parts.igem.org/Part:BBa_K431007

  6. afactor secretion signal: http://parts.igem.org/Part:BBa_K1800001

  7. BBa_I764001: http://parts.igem.org/Part:BBa_I764001

  8. BBa_K863203: http://parts.igem.org/Part:BBa_K863203:Design

  9. BBa_K805010: http://parts.igem.org/Part:BBa_K805010

Vectors

  1. pPICZa: https://www.ncbi.nlm.nih.gov/pubmed/24166764
  2. pPIC9K: https://www.ncbi.nlm.nih.gov/pubmed/19198937

Acknowledgements

This entry was curated by iGEM 2017 teams IISc-Bangalore and INSA-UPS France