Lactococcus lactis

prokaryotic facultative-anaerobic gram-positive lactose-fermenting

Maintenance Protocols

  1. Media and growth conditions for Lactococcus lactis :
    http://classrooms.tacoma.k12.wa.us/wilson/vrehberg/documents/download/agar_multitype_basic_protocols.pdf?id=145903

Transformation Protocols

  1. Efficient transformation:
    http://onlinelibrary.wiley.com/doi/10.1002/jobm.200610297/pdf

  2. Electrotransformation of L. lactis.:
    https://www.nature.com/protocolexchange/protocols/287#/procedure

Other Protocols

  1. VBEx (Vector backbone exchange) procedure:
    https://www.nature.com/protocolexchange/protocols/287#/procedure

Biobrick Parts

  1. BBa_K137009: https://openwetware.org/wiki/IGEM:Caltech/2008/Project/Vitamins

  2. BBa_K137011: https://openwetware.org/wiki/IGEM:Caltech/2008/Project/Vitamins

  3. BBa_K137005: https://openwetware.org/wiki/IGEM:Caltech/2008/Project/Vitamins

  4. BBa_K137006: https://openwetware.org/wiki/IGEM:Caltech/2008/Project/Vitamins

Vectors

  1. pSpeedET: http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=326951

  2. pSEUDO: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187151/

Metabolic Models

  1. Genome scale flux model: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-5-39