Bacillus subtilis

prokaryotic facultative-anaerobic gram-positive endosporulating

Culture sources

  1. ATCC: https://www.atcc.org/en/Products/Collections/Preceptrol_Cultures/23857.aspx#generalinformation
  2. Bacillus Genetic Stock Centre: http://www.bgsc.org/

Growth Media

  1. LB broth: http://2014.igem.org/wiki/images/5/59/LMU_Munich14_Media.pdf
  2. Difco Sporulation medium: http://2014.igem.org/wiki/images/5/59/LMU_Munich14_Media.pdf
  3. Chemically defined medium: https://file.scirp.org/pdf/AiM_2014061709514537.pdf
  4. Modified Chemically Defined Media: http://2014.igem.org/wiki/images/5/59/LMU_Munich14_Media.pdf

Transformation Protocols

  1. Efficient Transformation: https://link.springer.com/article/10.1007%2Fs00253-012-3987-2
  2. Requirements for transformation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC279084/pdf/jbacter00480-0093.pdf
  3. How To Bacillus subtilis: http://2016.igem.org/wiki/images/7/74/T--UBonn_HBRS--How-To-Bacillus-Subtilis.pdf

Other Protocols

  1. Cell disruption and extraction of RNA: http://subtiwiki.uni-goettingen.de/wiki/images/8/82/SOP-RNA.pdf
  2. Transpoon mutagenesis: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264109/

Biobrick Parts

  1. BBa_I742123: http://parts.igem.org/Part:BBa_I742123
  2. pBS1C: http://parts.igem.org/wiki/index.php?title=Part:BBa_J179000
  3. pBS2E: http://parts.igem.org/Part:BBa_J179001
  4. pBs4S: http://parts.igem.org/wiki/index.php?title=Part:BBa_J179002
  5. Episomal Vector with Constitutive GFP: http://parts.igem.org/wiki/index.php?title=Part:BBa_K090402
  6. Shuttle Vector for Chromosomal Integration: http://parts.igem.org/wiki/index.php?title=Part:BBa_K090403
  7. BBa_K1065204: http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204
  8. Integration vector for Bacillus subtilis derived from pSac-Cm: http://parts.igem.org/wiki/index.php?title=Part:BBa_K1185004
  9. pSBbs4E: http://parts.igem.org/wiki/index.php?title=Part:BBa_K1364021
  10. pSBBs1C-lacZ: http://parts.igem.org/wiki/index.php?title=Part:BBa_K823021
  11. pSBBs4S: http://parts.igem.org/wiki/index.php?title=Part:BBa_K823022
  12. pSBBs1C: http://parts.igem.org/wiki/index.php?title=Part:BBa_K823023
  13. pSBBs4S-P: http://parts.igem.org/wiki/index.php?title=Part:BBa_K823024
  14. pSBBs3C-luxABCDE: http://parts.igem.org/wiki/index.php?title=Part:BBa_K823025
  15. pSBBs2E: http://parts.igem.org/wiki/index.php?title=Part:BBa_K823027

Vectors

  1. PAC5: https://www.ncbi.nlm.nih.gov/pubmed/1619665
  2. PAC6: http://subtiwiki.uni-goettingen.de/wiki/index.php/PAC6
  3. PAC7: http://subtiwiki.uni-goettingen.de/wiki/index.php/PAC7
  4. pBaSysBioII: https://www.ncbi.nlm.nih.gov/pubmed/20150235

Metabolic Models

  1. iYO844: http://bigg.ucsd.edu/models/iYO844

Other online resources

  1. SubtiWiki: http://subtiwiki.uni-goettingen.de/

References