Acidithiobacillus ferrooxidans

prokaryotic facultative-anaerobic chemolithoautotrophic acidophilic metal-solubilizing gram-negative

Culture Sources

  1. ATCC 23270: https://www.atcc.org/products/all/23270.aspx#generalinformation

Maintenance Protocols

  1. ATCC Medium 2039: https://www.atcc.org/~/media/4C29EA2E2A0749BB9354D8AC64AA715B.ashx

  2. DSMZ Medium 70: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium70.pdf

  3. DSMZ Medium 71: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium71.pdf

  4. General Information: http://www.straininfo.net/taxa/4058

Transformation Protocols

  1. Electrotransformation of Thiobacillus ferrooxidans with Plasmids:
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC207636/pdf/jbacter00086-0309.pdf

Other Protocols

  1. Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay:
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548112/

  2. Comparative Genomics of the Extreme Acidophile Acidithiobacillus thiooxidans Reveals Intraspecific Divergence and Niche Adaptation:
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000751/

Biobrick Parts

  1. Iron oxidase generator: http://parts.igem.org/Part:BBa_K2055425

  2. Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron: http://parts.igem.org/Part:BBa_K2055014

  3. Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe:
    http://parts.igem.org/Part:BBa_K2055015

  4. Part designed for verification and characterization of the FUR/LacI inverter: http://parts.igem.org/Part:BBa_K2055016

  5. Tetrathionate Hydrolase (TetH) CDS with a RBS for overexpression in Acidithiobacillus ferrooxidans: http://parts.igem.org/Part:BBa_K2055017

  6. Heat Shock Promoter: http://parts.igem.org/Part:BBa_K2055013

  7. Heat shock promoter with RBS and RFP: http://parts.igem.org/Part:BBa_K2055018

  8. Part designed for verification and characterization of the heat the shock promoter and Inverter: http://parts.igem.org/Part:BBa_K2055019

  9. Overexpression of TetH with heat shock promoter and Inverter: http://parts.igem.org/Part:BBa_K2055020